Protein ID (NP or XP #) or Wolbachia#: XP_828627.1
Organism (including strain): trypanosoma brucei
Etiologic Risk Group (see link below): Risk Group 2: Bacterial agents
Disease Information: A protozoa of the speciestrypanosoma brucei (gambiense) causes African trypanosomiasis, a sleeping sickness, in humans. It is usually transmitted by the bite of an infected tsetse fly found most commonly in rural areas around sub-Saharan Africa. Symptoms include fevers, headaches, itchiness, joint pains, confusion, poor coordination, numbness and trouble sleeping. Diagnosis is via finding the parasite in a blood smear or in the fluid of a lymph node.
Amino Acid Sequence (paste as text only - not as screenshot or as 'code'): MSKPQPIAAANWKCNGSQQSLSELIDLFNSTSINHDVQCVVASTFVHLAMTKERLSHPKFVIAAQNAIAK SGAFTGEVSLPILKDFGVNWIVLGHSERRAYYGETNEIVADKVAAAVAAGFMVIACIGETLQERESGRTA VVVLTQIAAIAKKLKKADWAKVVIAYEPVWAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRILY GGSVNGKNARTLYQQRDVNGFLVGGASLKPEFVDIIKATQ length of your protein in Amino Acids: 250 amino acids
Molecular Weightof your protein in kiloDaltons using theExpasy ProtParamwebsite: 26818.8 kDa MolarExtinction coefficientof your protein at 280 nm wavelength: Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 35075 Abs 0.1% (=1 g/l) 1.308, assuming all pairs of Cys residues form cystines
Ext. coefficient 34950 Abs 0.1% (=1 g/l) 1.303, assuming all Cys residues are reduced
NCBI Gene # or RefSeq#: GI: 71755425
Protein ID (NP or XP #) or Wolbachia#: XP_828627.1
Organism (including strain): trypanosoma brucei
Etiologic Risk Group (see link below): Risk Group 2: Bacterial agents
Disease Information:
A protozoa of the speciestrypanosoma brucei (gambiense) causes African trypanosomiasis, a sleeping sickness, in humans. It is usually transmitted by the bite of an infected tsetse fly found most commonly in rural areas around sub-Saharan Africa. Symptoms include fevers, headaches, itchiness, joint pains, confusion, poor coordination, numbness and trouble sleeping. Diagnosis is via finding the parasite in a blood smear or in the fluid of a lymph node.
Link to TDR Targets page (if present):
http://www.tdrtargets.org/targets/view?gene_id=19362
Link to Gene Database page:
http://www.ncbi.nlm.nih.gov/protein/71755425
Essentiality of this protein:
Is it a monomer or multimer as biological unit? (make prediction at http://www.ebi.ac.uk/msd-srv/prot_int/pistart.html): monomer
Complex of proteins?: no
Druggable Target (list number or cite evidence from a paper/database showing druggable in another organism):
http://www.sciencedirect.com/science/article/pii/S0960894X0601211X
http://www.sciencedirect.com/science/article/pii/S0960894X05003677
EC#: 5.3.1.1
Link to BRENDA EC# page: http://www.brenda-enzymes.org/enzyme.php?ecno=5.3.1.1
Enzyme Assay information (spectrophotometric, coupled assay ?, reagents):
-- link to Sigma (or other company) page for assay (see Sigma links below):
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3138527/
-- link (or citation) to paper that contains assay information:
http://www.brenda-enzymes.org/literature.php?e=5.3.1.1&r=678177
-- links to assay reagents (substrates) pages:
D-Glyceraldehyde 3-phosphate = glycerone phosphate
--- List cost and quantity of substrate reagents, supplier, and catalog #:
D-Glyceraldehyde 3-phosphate: 8-13 mg/mL in H2O ,39705-1ML, $249.00, Sigma
Structure (PDB or Homology model)
PDB # or closest PDB entry if using homology model: 1AG1
Current Inhibitors: 2-phosphoglycolic acid (PGA), phosphoenolpyruvate (PEP)
Expression Information (has it been expressed in bacterial cells):Schistosoma japonicum
Purification Method: http://www.ncbi.nlm.nih.gov/pubmed/752201
Amino Acid Sequence (paste as text only - not as screenshot or as 'code'):
MSKPQPIAAANWKCNGSQQSLSELIDLFNSTSINHDVQCVVASTFVHLAMTKERLSHPKFVIAAQNAIAK
SGAFTGEVSLPILKDFGVNWIVLGHSERRAYYGETNEIVADKVAAAVAAGFMVIACIGETLQERESGRTA
VVVLTQIAAIAKKLKKADWAKVVIAYEPVWAIGTGKVATPQQAQEAHALIRSWVSSKIGADVAGELRILY
GGSVNGKNARTLYQQRDVNGFLVGGASLKPEFVDIIKATQ
length of your protein in Amino Acids: 250 amino acids
Molecular Weight of your protein in kiloDaltons using the Expasy ProtParam website: 26818.8 kDa
Molar Extinction coefficient of your protein at 280 nm wavelength:
Extinction coefficients are in units of M-1 cm-1, at 280 nm measured in water.
Ext. coefficient 35075
Abs 0.1% (=1 g/l) 1.308, assuming all pairs of Cys residues form cystines
Ext. coefficient 34950
Abs 0.1% (=1 g/l) 1.303, assuming all Cys residues are reduced
TMpred graph Image (http://www.ch.embnet.org/software/TMPRED_form.html:
CDS Gene Sequence (paste as text only):
atgagcaaaccgcagccgattgcggcggcgaactggaaatgcaacggcagccagcagagc
ctgagcgaactgattgatctgtttaacagcaccagcattaaccatgatgtgcagtgcgtg
gtggcgagcacctttgtgcatctggcgatgaccaaagaacgcctgagccatccgaaattt
gtgattgcggcgcagaacgcgattgcgaaaagcggcgcgtttaccggcgaagtgagcctg
ccgattctgaaagattttggcgtgaactggattgtgctgggccatagcgaacgccgcgcg
tattatggcgaaaccaacgaaattgtggcggataaagtggcggcggcggtggcggcgggc
tttatggtgattgcgtgcattggcgaaaccctgcaggaacgcgaaagcggccgcaccgcg
gtggtggtgctgacccagattgcggcgattgcgaaaaaactgaaaaaagcggattgggcg
aaagtggtgattgcgtatgaaccggtgtgggcgattggcaccggcaaagtggcgaccccg
cagcaggcgcaggaagcgcatgcgctgattcgcagctgggtgagcagcaaaattggcgcg
gatgtggcgggcgaactgcgcattctgtatggcggcagcgtgaacggcaaaaacgcgcgc
accctgtatcagcagcgcgatgtgaacggctttctggtgggcggcgcgagcctgaaaccg
gaatttgtggatattattaaagcgacccag
GC% Content for gene: 57.73 %
CDS Gene Sequence (codon optimized) - copy from output of Primer Design Protocol (paste as text only):
GC% Content for gene (codon optimized):